Tue. 19 June 2018: 14:00
Room: Room 27
In this symposium speakers will address the role of contact lens disinfection in maintaining the hygiene of contact lenses, and the issues that occur when the disinfection systems are not used appropriately. The session will cover how contact lens disinfecting systems are involved in the epidemiology of microbial keratitis, the role of the contact lens case in keratitis. New regulartory guidelines developed to reduce the risk involved in using these sysyems, and new disinfecting systems that improve antimicrobial efficacy and reduce the trasnfer of microbes onto contact lenses. The session will also examine new antimicrobial contact lenses are their role in improving the safety of contact lens wear
1. Knowledge on the risks involved in non-compliance with contact lens disinfecting systems
2. Knowledge of new guidelines on the manufacture and use of contact lens disinfecting systems
3. Knowledge on new ways of improving the safety of contact lens wear.
Contact Lens and Refraction
The use of soft contact lenses (CL), one of the most popular ways to correct vision disorders, accounts for approximately 12%–66% of all microbial keratitis cases (1). This is mainly due to the colonization of lenses by microorganisms, especially the multidrug resistant and biofilm forming opportunistic bacterial pathogen Pseudomonas aeruginosa.
Importantly, novel strategies and compounds to reduce CL-associated ocular infections are needed. Recently, the frog skin-derived antimicrobial peptide Esc(1-21) and its diastereomer Esc(1-21)-1c resulted to have a bactericidal activity against both planktonic and sessile forms of the aforementioned pathogen. Furthermore, Esc(1-21) was found to significantly reduce the severity of P. aeruginosa keratitis in a mouse model and to preserve antipseudomonal activity in the presence of human basal tears (2).
In this work, we demonstrated that both peptides are able to disrupt P. aeruginosa biofilm formed on soft CLs, with the diastereomer having the greater efficacy. In addition, upon covalent immobilization to the CL, the two peptides caused more than four log reduction in the number of bacterial cells within 20 minutes and reduced bacterial adhesion to the CL surface (77%–97% reduction) in 24 hours. Importantly, peptide immobilization was not toxic to mammalian cells and did not affect the lens surface parameters (3). Overall, these data suggest that both peptides have great potential to be developed as novel pharmaceuticals for prevention and treatment of CL-associated P. aeruginosa keratitis.
1. D. Dutta, N. Cole, M. Willcox, Mol. Vis. 2012, 18, 14
2. S. S. Kolar, V. Luca, H. Baidouri, G. Mannino, A. M. McDermott, M.L. Mangoni, Cell Mol. Life Sci. 2015, 72, 617.
3. B. Casciaro, | D. Dutta, MR Loffredo, S. Marcheggiani, AM McDermott, MDP Willcox, ML Mangoni. Biopolymers, 2017, doi: 10.1002/bip.23074